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To give some guidance toward selecting an appropriate threshold, we show here the results of different thresholds on the MiSeq metagenome from the Kraken paper see the paper for more details; note that the database used here is more recent than that used in the paper. The fields of the output, from left-to-right, are as follows:. A full list of options for kraken-build can be obtained using kraken-build --help.
The files containing the sequences to be classified should be specified on the command line. To run efficiently, Kraken requires enough free memory to hold the database in RAM. Building the standard Kraken database downloads and uses all complete bacterial, archeal, and viral genomes in Refseq at the time of the build.